Figure 2.

Identification of a novel LXRE site downstream pf FXRE in the hamster SR-BI gene. (A) Putative cis-regulatory elements located in the hamster site B luciferase reporter construct. (B) Reporter constructs were transiently cotransfected with pRL-TK vector into HepG2 cells. The following day, cells were cultured in MEM containing 0.5% fetal bovine serum overnight and treated with GW4064 (1 µM), OCA (10 µM), or LXR agonist GW3965 (5 µM) for 24 h prior to cell lysis. Data are presented as the mean ± standard error of the mean of four replicates per treatment and are expressed as the ratio of firefly/Renilla activity from each sample where the relative luminescence from DMSO-treated cells is set to 1. Statistical significance among all groups was assessed by one-way analysis of variance with Tukey's multiple comparison test. ^*P<0.05 and ^***P<0.001 compared with DMSO-treated samples. The data shown are representative of three separate transfection experiments. FXR, farnesoid X receptor; FXRE, FXR response element; LXR, liver X receptor; LXRE, LXR response element; SR-BI, scavenger receptor class B type I; OCA, obeticholic acid.