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. 2019 Mar 18;43(5):1927–1938. doi: 10.3892/ijmm.2019.4136

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Copyright: © Dong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Synergistic activation of the gene expression of SR-BI by farnesoid X receptor and liver X receptor in HPH and HepG2 cells. (A) HPHs were treated with OCA (10 µM) or GW3965 (1 µM) or OCA + GW3965 for 24 h prior to isolation of total RNA. Triplicate wells were used in each treatment condition. RT-qPCR analysis was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. Statistical signifi-cance among all groups was assessed by one-way ANOVA with Tukey's multiple comparison test. ^**P<0.01 and ^***P<0.001 compared with DMSO-treated samples; ^##P<0.01 and ^###P<0.001 compared with cotreated samples. (B) HepG2 cells in triplicate wells were cultured overnight in culture medium containing 0.5% fetal bovine serum, followed by treatment of OCA (10 µM), GW3965 (5 µM), or GW3965 + OCA for 24 h. RT-qPCR analysis was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. One-way ANOVA with Tukey's multiple comparison test was used. HPH, human primary hepatocytes; OCA, obeticholic acid; GW, GW3965; SR-BI, scavenger receptor class B type I; ABC, ATP binding cassette; ANOVA, analysis of variance; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Synergistic activation of the gene expression of SR-BI by farnesoid X receptor and liver X receptor in HPH and HepG2 cells. (A) HPHs were treated with OCA (10 µM) or GW3965 (1 µM) or OCA + GW3965 for 24 h prior to isolation of total RNA. Triplicate wells were used in each treatment condition. RT-qPCR analysis was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. Statistical signifi-cance among all groups was assessed by one-way ANOVA with Tukey's multiple comparison test. ^**P<0.01 and ^***P<0.001 compared with DMSO-treated samples; ^##P<0.01 and ^###P<0.001 compared with cotreated samples. (B) HepG2 cells in triplicate wells were cultured overnight in culture medium containing 0.5% fetal bovine serum, followed by treatment of OCA (10 µM), GW3965 (5 µM), or GW3965 + OCA for 24 h. RT-qPCR analysis was performed to measure relative mRNA levels of indicated genes with duplicate measurement of each cDNA sample. One-way ANOVA with Tukey's multiple comparison test was used. HPH, human primary hepatocytes; OCA, obeticholic acid; GW, GW3965; SR-BI, scavenger receptor class B type I; ABC, ATP binding cassette; ANOVA, analysis of variance; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.