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Induction of autophagy is associated with the expression of Atg5. Immunofluorescence of LC3 was detected in the four H9c2 cell groups (scale bar, 10 µm). NC, normal H9c2 control cells; H/R, H9c2 cells under H/R; siRNA-Con, H9c2 cells transfected with control siRNA; Atg5-siRNA + H/R, H9c2 cells transfected with Atg5-siRNA and then H/R. miR, microRNA; H/R, hypoxia/reoxygenation; NC, negative control; siRNA, small interfering RNA; Atg5, autophagy-related 5.
