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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Am Chem Soc. 2017 Aug 21;139(34):11734–11744. doi: 10.1021/jacs.7b01283

Figure 2.

Figure 2

MS analysis of CteA modified by CteB. a) Expected masses of CteA modified by CteB and alkylated with NEM. b) MS of CteA modified by CteB. In red is 1 eq. of CteA treated with 1 eq. of CteB while in black is CteA unmodified. The difference between corresponding charge states is that of one sactionine bridge or two hydrogen atoms. c) MS of CteA product alkylated with NEM after first being modified by CteB. In red is 1 eq. of CteA treated with 1 eq. of CteB then NEM, while in black is CteA treated with just NEM. The difference between corresponding charge states is that of one sactionine bridge and one NEM modification. See Table S3 for expected exact masses. d) MS/MS analysis (+7 charge state) of where the sactionine bridge is forming in modified CteA.