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MIEF1-MP is a mitochondrial matrix microprotein. (A) MIEF1-MP-FLAG colocalizes with the mitochondrial marker TOM20 in Hela cells verifying its mitochondrial localization (Hoechst (blue) nuclear stain, TOM20 (red), MIEF1-MP (green), yellow/orange overlap). (B) Proteinase K digestion of MIEF1-MP-FLAG had a similar pattern to HSP60, a mitochondrial matrix marker, confirming its sub-cellular localization to the mitochondrial matrix in HEK293T cells.
