Table 2:
Antibodies used for immunoblot analysis
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclona l or Polyclonal | Dilution Used | RRID |
|---|---|---|---|---|---|---|
| Bovine Adiponectin | Bovine anti-adiponectin | Dr. Helga Sauerwein (University of Bonn, Germany) | Rabbit; polyclonal | 1:1000 | AB_2650604 | |
| CYP17 | Rabbit anti-17α-hydroxylase | Dr. Walter L Miller (University of California, San Francisco) | Rabbit; polyclonal | 1:1000 | AB_2732802 | |
| CYP19 | Aromatase Polyclonal Antibody | Thermo Fisher Scientific, PA1–21398 | Rabbit; polyclonal | 1:1000 | AB_2088676 | |
| AR | AR (N-20) | Santa Cruz Biotechnology, SC-816 | Rabbit; polyclonal | 1:1000 | AB 1563391 | |
| ESR1 | ERα (clone 1D5) | Thermo Scientific, MS-354 | Mouse; monoclonal | 1:500 | AB 61341 | |
| ESR2 | Estrogen receptor beta (ER 2) antibody | Dr. Benita S Katzenellenbog en (University of Illinois) | Mouse; monoclonal | 1:1000 | AB_2722105 | |
| GAPDH | GAPDH (14C10) | Cell Signaling, 3683 | Rabbit; monoclonal | 1:1000 | AB 1642205 |