Table I.
Cells within corneal epithelial sheets exhibit differences in movement during retraction and forward motility on 8 kPa and 30 kPa substrates.
| 8 kPa | 30 kPa | |||||
|---|---|---|---|---|---|---|
| Retraction (μm) | X | Y | Z | X | Y | Z |
| LE | 2.1+/.15 | 2.5+/−.16 | 3.3 | 4.6+/−.14 | 2.6+/.3 | 5.3 |
| BFLE | 10.1+/−.94 | 3.1+/−.19 | 10.6 | 6.8+/−.2 | 15+/−3.3 | 16.5 |
| Forward (μm) | X | Y | Z | X | Y | Z |
| LE | 1.6+/−.3 | 1.5+/−.3 | 2.2 | 3+/−.3 | 7.1+/−.2 | 7.7 |
| BFLE | 1.6+/−.3 | 1.3+/−.2 | 2.1 | 4.9+/−.1 | 4.7+/−.3 | 6.8 |
Epithelial sheets were removed from rat corneas and placed onto fibronectin-coated 8 kPa and 30 kPa polyacrylamide gels. Images were obtained every 10 minutes for 12 hours using the 10x objective on a Zeiss LSM 880 confocal microscope. A minimum of 5 epithelial sheets were imaged per experiment with a minimum of 5 experiments. Magnitude of bead displacement or hypotenuse (∆Z) was obtained by measuring the change in X (∆X) and Y (∆Y) coordinates of individual beads throughout the course of experimentation. SEM is calculated for the change in x and y. Analysis was performed using FIJI/ImageJ (NIH, Bethesda, MD; http://imagej.nih.gov/ij/).