. Author manuscript; available in PMC: 2019 Apr 1.

Published in final edited form as: Nat Biotechnol. 2018 Oct 1:10.1038/nbt.4262. doi: 10.1038/nbt.4262

Table 2.

List of buffers used within the InSCyT system

Buffer number	Buffer component	Concentration (mM unless indicated)	pH	Conductivity (mS/cm)
1	Sodium citrate	20	5.5	4
2	Sodium phosphate	20	6	4
3	Sodium phosphate	20	8	4
4	Sodium hydroxide	1000	-	-
5	Sodium citrate	10	6	-
5	Sodium chloride	8.7 g/L	6	-
6	Sodium citrate	10	6	-
	Sodium chloride	8.7 g/L
	Tween 20	0.2% v/v
7	Sodium citrate	20	5	-
8	Sodium phosphate	20	6.6	-
9	Trisodium citrate	200	7.6	-
	Sodium chloride	100
	Sodium phosphate	20
10	Sodium citrate	20	5.6	-
11	Sodium citrate	20	4	-
12	Sodium chloride	240	4	-
12	Sodium citrate	10	4	-
13	Sodium chloride	410	4	-
13	Sodium citrate	10	4	-
14	Sodium chloride	7.5 g/L	6.75	-
	Sodium phosphate dibasic	1.8 g/L
	Sodium phosphate monobasic	1.3 g/L
15	Sodium chloride	400	4	-
15	Sodium citrate	10	4	-
16	Sodium phosphate	40	7.6	-
16	Sodium chloride	200	7.6	-
17	Sodium citrate	20	4.2	-
18	Sodium citrate	20	3.5	-
19	Sodium chloride	7.5 g/L	6.75	-
	Sodium phosphate dibasic	1.8 g/L
	Sodium phosphate monobasic	1.3 g/L
	EDTA	0.1 g/L
	Tween 80	0.1 g/L
20	Sodium chloride	150	5.8	-
20	Sodium phosphate	20	5.8	-
21	Sodium chloride	150	7	-
21	Sodium phosphate	20	7	-
22	Sodium citrate	20	5.5	-
23	Glutamic acid	10	4.4	-
Sobitol	5% w/v