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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Nat Biotechnol. 2018 Oct 1:10.1038/nbt.4262. doi: 10.1038/nbt.4262

Table 4.b:

RPLC operating conditions for IFNα-2b

Time (min) Flow (mL/min) %A %B
Initial 0.5 100 0
0.1 0.5 100 0
0.5 0.5 61 39
5.5 0.5 54.5 45.5
5.75 0.5 0 100
6 0.5 0 100
7.5 0.5 100 0

Column Temperature: 60°C