Figure 1:

Outline of the study design. The dosing consisted of a regimen of 4 unit doses of 80 mg/kg of α-PPP or saline administered over an 8 hour period with each dose separated by 2 hours. The dosing regimen was administered on day 1, and its persistent effects on anxiety, memory, and tissue monoamine neurochemistry were assessed as presented. Two separate groups of mice were assessed. Group one underwent dosing with α-PPP (80 mg/kg, QID, q2h, IP) and was assessed in the Y-maze and elevated plus maze (EPM) four days later, with EPM assessments occurring at least three hours after the Y-maze assessments. Group two underwent dosing with α-PPP (80 mg/kg, QID, q2h, IP) and was assessed in the NOR test four days later and sacrificed for neurochemical analysis the following day.