Figure 2.
Western blot (A and B) and densitometry analysis (C and D) of CBS and CSE expression in normal canine, NHP and human retinas. CBS and CSE protein expression was normalized with GAPDH (housekeeping protein) and expressed as fold increase over human samples. CBS protein levels in the NHP samples were higher than canines and human. Canine retina also showed higher level of CBS protein expression compared to human samples. CSE protein levels in the canine retina samples were higher than NHP and human. The results are shown as the means ± SD. (One-way ANOVA, **= p< 0.01 and * =p< 0.05). Protein samples were prepared from 3 different animals and human and the experiment was repeated 3 times, representative levels are illustrated.