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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Exp Eye Res. 2019 Jan 14;181:72–84. doi: 10.1016/j.exer.2019.01.007

Figure 3.

Figure 3.

CBS and CSE antibody validation using blocking peptides kit and isotype match antibody in western blot and IHC. Western blot was perform with and without blocking peptide to determine CBS (A and B) and CSE (C and D) antibodies specificity. Results showed that there wasn’t any band on the blot when blocking peptide was used (B,D) while corresponding bands for CBS or CSE was detected on the blots using primary antibodies without blocking peptides (A, C). IHC results also confirmed specificity of the CBS and CSE antibodies used in this experiment. Results showed that CBS and CSE proteins were not detected when corresponding isotype controls were used for CBS (F) and CSE (H) while CBS (E) and CSE (G) protein were detected using primary antibodies.