Table 3.
Antibodies used for specific cell markers in immunohistochemistry
| Antigen | Antibody | Host | Type | Localized cells | Dilution | Reference |
|---|---|---|---|---|---|---|
| RPE65 | J.M Nickerson U of Georgia |
Rabbit | IgG polyclonal |
RPE | 1:1000 | (Guziewicz et al., 2018; Vecino et al., 2016) |
| hCAR (Human cone arrestin) |
W, Beltran, U of Pennsylvania |
Goat | IgG Polyclonal |
Cones | 1:50 | (Kondo et al., 2015) |
| Rhodopsin | ||||||
| Millipore MABN15 |
Mouse | IgG2b Monoclonal |
Rods | 1:500 | (Cideciyan et al., 2018) | |
| Ezrin | ||||||
| Abcam ab4069 |
Mouse | IgG1 Monoclonal |
RPE microvilli | 1:500 | (Guziewicz et al., 2018) | |
| PNA (Peanut agglutinin) |
Invitrogen L32458 |
Lectin |
______ |
Cone extracellular matrix |
1:50 |
(Guziewicz et al., 2018) |
| WGA (wheat germ agglutinin) |
Invitrogen W11262 |
______ |
Rod extracellular matrix |
1:100 |
(Hauzman et al., 2017) |
|
| Parvalbumin | Millipore MAB1572 |
Mouse | IgG1 Monoclonal |
Horizontal and AII amacrine cells |
1:1000 | (Roski et al., 2018) |
| Neurofilament 200KDa |
Millipore MAB5266 |
Mouse | IgG1 Monoclonal |
GCL/NFL | 1:200 | (Singh et al., 2014; Vecino et al., 2016) |
| PKCα | Millipore MAB302 |
Mouse | IgG2a Monoclonal |
Rod bipolar cells | 1:1000 | (Singh et al., 2014; Vecino et al., 2016) |
| GAD65 | R&D Systems, AF2247 |
Goat | IgG Polyclonal |
GABAergic amacrine cells |
1:100 | (Schubert et al., 2010) |
| GS (Glutamine Synthetase |
BD Bioscience, 610107 |
Mouse | IgG2b Monoclonal |
Müller cells | 1:100 | (Sudharsan et al., 2017) |