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. 2019 Mar 5;41:670–682. doi: 10.1016/j.ebiom.2019.02.057

Fig. 6.

Fig. 6

miR-4739 over-expression results in pleural fibrosis in mice. (a) Sequences of has-miR-4739 and the putative target sequence in the mouse or rat BMP-7 mRNA (wild-type) or an engineered mutant of this sequence (mutant) for the luciferase activity assay. n = 3, *P < 0.05 versus wild-control; #P < 0.05 versus mutant-control (ANOVA test). (b) Rat primary PMCs were transducted with recombinant lentivirus vector containing miR-4739 or scrambled negativecontrol (vector control) plasmids, and then treated with or without BMP-7 (0.1 μg/ml) for 24 h. The protein levels of collagen-I, α-SMA, vimentin, and E-cadherin were detected. n = 3, *P < 0.05 versus vector control, #P < 0.05 versus miR-4739 over-expression group (ANOVA test). (c-e) C57BL/6 mice were treated with miR-4739 lentivirus plasmids or vector control lentivirus plasmids and carbon particles by intrapleural injection at days 1, 5 and 8. All mice were euthanized at day 21, and then tissues were taken for histological staining. (c) Masson's trichrome staining of visceral pleura, parietal pleura and diaphragmatic pleura. (d) Bar graphs depicting changes of pleura thickness. n = 3, #P < 0.05 versus vector control (Student's t-test). (e) Bar graphs depicting changes of pleura collagen percentage. n = 3, #P < 0.05 versus vector control (Student's t-test).