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. 2019 Mar 5;41:670–682. doi: 10.1016/j.ebiom.2019.02.057

Fig. 7.

Fig. 7

BMP-7 reserved bleomycin induced pleural fibrosis in mice. (a-b) Rat primary PMCs were treated with or without bleomycin (0.2 μg/ml) in the presence or absence of BMP-7 (0.1 μg/ml) for 24 h. (a) Changes of protein levels of p-Smad2/3 and p-Smad1/5/9 were measured by Western blotting. n = 3, *P < 0.05 versus control, #P < 0.05 versus bleomycin group (ANOVA test). (b) Changes of protein levels of collagen-I (n = 4), E-cadherin (n = 3), vimentin (n = 3) and α-SMA (n = 4) were measured by Western blotting. *P < 0.05 versus control, #P < 0.05 versus bleomycin group (ANOVA test). (c-e) C57BL/6 mice were treated with a mixture of bleomycin and carbon particles or PBS by intrapleural injection. At days 4, 7 and 10, mice were treated with BMP-7 by intra-pleural injection. All mice were euthanized at day 21, and then tissues were taken for histological staining. (c) Masson's trichrome staining of visceral pleura, parietal pleura and diaphragmatic pleura. (d) Bar graphs depicting changes of pleura thickness. n = 4, *P < 0.05 versus control, #P < 0.05 versus bleomycin group (ANOVA test). (e) Bar graphs depicting changes of pleura collagen percentage. n = 4, *P < 0.05 versus control, #P < 0.05 versus bleomycin group (ANOVA test).