Skip to main content
. 2019 Mar 26;10:600. doi: 10.3389/fmicb.2019.00600

FIGURE 2.

FIGURE 2

Expression of CRF1 alternative transcripts in A. fumigatus. (A) Schematic representation of CRF1 gene and the three mRNA transcripts, Crf1, Crf2, and Asp f9 with the corresponding primers used for amplification. Nucleotide positions of the beginning/end of each exon (boxes) and intron (Int., in full lines) are mentioned under the gene and the transcripts. Non-coding regions are identified in dotted lines. SP: position of predicted signal peptide. CS: position of predicted catalytic site. GPI: position of predicted GPI anchor. Position of primers used for the amplification of the different transcripts is indicated by gray arrows (>). (B) In vitro expression of Crf1, Crf2 and Asp f9 RNA transcripts in RT-qPCR. Transcript expression was analyzed in Crf positive strain by RT-qPCR and normalized toward gpdA (glutaraldehyde 3-phosphate dehydrogenase) and TUB1 (beta-tubulin) housekeeping genes, set to 1. Results (n = 5) are expressed in mean ± SD.