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Growth of CRF1 engineered mutant strain in vitro. (A) Assessment of the consequences of the absence of CRF1 gene (coding Crf1, Crf2, and Asp f9 proteins) on fungal growth. A growth kinetic was realized on Ku80 and Ku80 ΔCRF1 strains with a reading of 530 nm absorbance every 30 min, during 48 h. (B) Growth differences at 48 h. Results (n = 15) are expressed in mean ± SEM; Mann–Whitney statistical test was used. ∗∗∗p < 0.001.
