Fig. 3. Srlp overexpression increases proliferation ability and cell death in Drosophila S2 cells.

a Immunostaining of control and Srlp-overexpressing S2 cells using anti-PH3 (red) and Hoechst-33342 (blue). b Percentage of PH3-positive cells in the control and Srlp-overexpression group. c Immunostaining of the control and Srlp-overexpressing S2 cells by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling) (red) and Hoechst-33342 (blue). d Percentage of TUNEL-positive cells in the control and Srlp-overexpression groups. e Flow cytometry of control and Srlp-overexpressing cells. The ratio of viable cells dramatically decreased while that of apoptosis cells significantly increased. f Cell counting kit-8 (CCK-8) assay of the control and Srlp-overexpressing S2 cells. Student’s t-test was used for the statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant. Error bars represent SEM. Scale bar: 30 μm