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. 2019 Mar 26;10:602. doi: 10.3389/fmicb.2019.00602

FIGURE 1.

FIGURE 1

Results of chitosan treatment screening of the transcription factor and cell surface-related gene knockout library. Each mutant strain was separately grown in RPMI 1640 liquid medium (RPMI), RPMI containing 0.5% acetic acid (RPMI + buffer) or RPMI containing both 0.5% acetic acid and chitosan (RPMI + buffer + 0.5% chitosan) in 96-well microplates. The microplates were incubated at 37°C for 48 h, and the optical density of the cells was measured at 600 nm. The absorbance of each mutant strain treated with chitosan or acetic acid was compared to determine the susceptibility of the strains to chitosan. Statistical significance was determined by Student’s t-test (unpaired, two-tailed). ∗P < 0.05; ∗∗P < 0.01.