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. 2019 Mar 26;10:602. doi: 10.3389/fmicb.2019.00602

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Copyright © 2019 Shih, Liao, Tseng, Deng and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C. albicans ada2Δ strains were highly sensitive to challenge with chitosan. (A) The deletion of ADA2 resulted in a mild growth defect in the RPMI and buffer control groups (0.2% acetic acid) and completely abolished growth in 0.2% chitosan. Growth and chitosan resistance were rescued in the revertant strains. (B) Overnight cultures of C. albicans cells were diluted to an OD₆₀₀ of 0.1 in fresh YPD liquid medium. Growth rates were monitored with a Biowave density meter. The experiments were performed in biological triplicate.