Figure 1.

hASCs dependent self-assembly of HUVECs depends on the culture medium. 3D hydrogel constructs containing GFP positive HUVECs and hASCs after 2 days (a–c), 4 days (d–f), 7 days (g–i), and 9 days (j–l) of cultivation in EGM-2 (a,d,g,j), VSM (b,e,h,k), and SFM (c,f,i,l). Endothelial cell network formation starts between day 2–4 of cultivation under all cultivation conditions. From day 7 onwards endothelial cell networks start to collapse in VSM leading to loss of almost all GFP-HUVEC by day 9. EGM-2: endothelial growth medium-2, VSM: vascular stimulation medium, SFM: serum-free medium, Scale bar: (a–l): 500 µm