Table 1.
Comparison of network parameters.
| SFM/Matrigel/rCOL | SFM/hCOL | Significance | |
|---|---|---|---|
| Mean Diameter (µm) | 9.3 ± 2.4 | 12.2 ± 3.3 | ns |
| Number of nodes | 113.0 ± 19.3 | 81.7 ± 4.0 | ns |
| Total branching length (µm) | 10747.0 ± 938.9 | 8357.3 ± 526.6 | * |
| Network area (µm2) | (3.2 ± 0.3)*105 | (3.2 ± 0.1)*105 | ns |
| Network volume (µm3) | (7.7 ± 1.0)*105 | (10.4 ± 0.8)*105 | * |
| Number of segments | 207.3 ± 34.6 | 146.3 ± 4.2 | * |
Constructs containing GFP-HUVECs and hASCs and Matrigel/rCOL or hCOL were cultivated in SFM. After 14 days of cultivation multiphoton images were taken and processed with Imaris. Data is presented as mean ± standard deviation (SD) for the following parameter: mean diameter, number of nodes, total branching length (sum of the lengths of all segments within the 3D network), network area (the sum of areas of all segments within the 3D network), network volume (the sum of volumes of all segments within the 3D network), and number of segments. Data was analyzed with unpaired t-test. (*) p-value ≤ 0.05, ns: not significant.