Fig. 2.

Activation of signal transducer and activator of transcription factor 3 in colorectal tumors from Tg-tRXRα mice. a, b Colon lysates from mice treated with azoxymethane (AOM)/dextran sodium sulfate (DSS) for 120 days (a) or DSS for 6 months (b) were analyzed by immunoblotting. The bands are quantified with Image J and normalized to β-actin. c Colon sections from mice treated with AOM/DSS for 120 days or DSS for 6 months were stained with pSTAT3 antibody and analyzed the staining score. Scale bars, 25 μm. Data are mean ± SEM (n = 9), t test. d Body weight curve of control and Tg-tRXRα mice during the course of acute DSS-induced colitis. Data are mean ± SEM (n = 8), two-way analysis of variance. e Colon length from mice after the course of acute DSS-induced colitis. Data are mean ± SD (n = 8), t test. f Colonic lysates prepared from mice treated with DSS for the indicated time were analyzed by immunoblotting. g RegIIIγ and Tff3 mRNA expression in mice treated with DSS for two days was analyzed by quantitative reverse transcriptase–PCR. Data are mean ± SEM (n = 3), t test. *P < 0.05, **P < 0.01, ***P < 0.001. For immunoblotting, one of three or four similar experiments is shown