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. 2019 Apr 1;9:5399. doi: 10.1038/s41598-019-41782-1

Figure 3.

Figure 3

CatK cannibalized catL when co-incubated in the absence of substrate. Zymography (A) Immunoblot for catK (B) or catL (C) are shown. Densitometry quantified zymo active catK (D) catL €, or immunoblot for catK (F) catL >37 kDa (G) and catl <37 kDa (H). CatK and catL were incubated without substrate for 1, 4, and 8 hrs either alone, concurrently (+), or sequentially (→). Zymography showed active catK and catL remaining after incubation (A). CatK and catL are active at 1 hr, with 4 hr and 8 hr time points showing decreasing signal. Significantly more catK was active when concurrently co-incubated with catL (+) compared to catK alone and sequential co-incubation of catK then catL (→). However, active catL significantly decreased over time when co-incubated with catK. Significantly more catK was detected by Western blot when co-incubated with catL compared to catK alone. CatL amount significantly decreases over time when co-incubated with catK, and even when catL was added sequentially, suggesting catK cannibalism of catL. (n = 3, *p < 0.05 across conditions, #indicates statistical significance from the corresponding 1 hr bar within the same condition, p < 0.05 as determined by a one-way ANOVA. Error bars represent SEM.