Table 2.
Thermodynamic parameters of conformational stability of the wild-type and H59A mutant at 37 °C.
| pH | Wild-type | H59A | ||||||
|---|---|---|---|---|---|---|---|---|
| ∆Gu* | m–value† | ∆G1* | m1† | ∆G2* | m2† | ∆GU* | m† | |
| 5.0 | 6.1 ± 0.5 | 1.7 ± 0.1 | 7.9 ± 0.9 | 3.0 ± 0.1 | 5.0 ± 0.4 | 1.4 ± 0.1 | 17.9 | 5.8 |
| 5.5 | 6.3 ± 0.2 | 1.8 ± 0.1 | — | — | — | — | — | — |
| 6.0 | 6.9 ± 0.5 | 2.0 ± 0.1 | 8.6 ± 0.9 | 3.2 ± 0.2 | 5.6 ± 0.7 | 1.6 ± 0.2 | 19.8 | 6.4 |
| 7.0 | 8.4 ± 0.4 | 2.2 ± 0.1 | — | — | — | — | — | — |
| 7.8 | 8.9 ± 0.4 | 2.3 ± 0.1 | 8.2 ± 1.2 | 3.0 ± 0.1 | 6.0 ± 1.0 | 1.8 ± 0.1 | 20.2 | 6.6 |
*Expressed as kcal·mol−1.
†Expressed as kcal·mol−1·M−1.