Figure 14.

ASPD Accumulate Only in Excitatory Neurons

APPswe-transduced neuronal cultures were treated with 75 nM MG132 for 24 h and stained with the indicated antibodies. The ratio of the ASPD-containing neurons among either MAP2/PV or MAP2/Math2 double-positive neurons was quantified using a Yokogawa CQ1 (see Transparent Methods; inset, mean ± SD, n = 6 for MAP2/Math, 24 for MAP2/PV). ASPD were detected by anti-ASPD rpASD1 or mASD3 antibody. GABAergic interneurons are composed of a highly diverse population of inhibitory neurons, which represent about 20% of cortical/hippocampal neurons (Amaral, 1978). For quantification purposes, we used an antibody against PV, because PV-labeled interneurons represent ∼40% of the total number of GABAergic interneurons in the CA1 and CA3 of hippocampus (Ribak et al., 1990) and the PV antibody clearly stains the neuronal cell body. Representative images, used for the quantification, are shown with 100 μm scale bars. See control data in Figures S16 and S17 and calbindin data in Figure S18. See Figures S16–18.