Figure 8.

ASPD Are Also Present in the Recycling Endosomes of Mature Neurons

Primary rat hippocampal neuronal cultures, with or without AAV-APPswe transduction, were treated with 75 nM MG132 for the indicated time. The cultures were double-stained with anti-ASPD rpASD1 antibody and anti-transferrin receptor (TfR) antibody (see Transparent Methods). Representative images obtained with a highly sensitive, direct photon-counting system with a 100X oil objective lens are shown. An enlarged view of the field enclosed by a hatched line is shown below. Solid and hatched scale bars, 10 and 1 μm, respectively. Single red and green images for each time point are shown in Figure S6. See Figure S6.