Figure 4.

Inhibition of vitamin E biosynthesis in parasites treated with UA or NTBC for in vitro cultures of the Leishmania (L.) amazonensis promastigotes. (A) Radioactive elution profile of promastigotes treated (white bars) or untreated (grey bars) for 72 h with UA and labelled with [1‐(n)‐³H]‐phytol in the last 24 h. α‐T: α‐tocopherol, γ‐T: γ‐tocopherol, ?: unknown. (B) Radioactive elution profile of promastigotes treated (white bars) or untreated (grey bars) for 48 h with NTBC and labelled with [1‐(n)‐³H]‐phytol in the last 24 h. α‐T: α‐tocopherol, γ‐T: γ‐tocopherol, ?: unknown. Data are representative of two independent experiments. c.p.m.: count per minute.