Figure 6.

FGF11 overexpression did not affect the levels of phosphorylation of FGFRs. (A) FLAG‐tagged FGF11 was overexpressed by adenoviral expressing system (600 MOI) from day 2 to 4 of adipogenesis. Protein samples were prepared for western blot. (B) The density of phosphorylated FGFR1 (pFGFR1)/FGFR1 or pFGFR total/total FGFRs in (A) was quantified. The level from GFP‐treated cells was set to 1, and the other was calculated relative to this value (lower panel) (n = 3, average ± SD). (C) Fully differentiated cells were transduced with 600 MOI of FLAG‐tagged FGF11 expressing adenoviruses (FGF11) or control adenoviruses. After 2 days, protein samples were prepared and subjected to western blot. (D) The density of pFGFR1/FGFR1 or pFGFR total/FGFRs in (C) was quantified. The level from GFP‐treated cells was set to 1, and the other was calculated relative to this value (lower panel) (n = 3, average ± SD).