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. 2019 Mar 18;9(4):755–768. doi: 10.1002/2211-5463.12616

Figure 2.

Figure 2

Binding activities of LC‐VNs toward collagen. (A) Collagen type I was immobilized in wells of 96‐well plates. Normal and LC plasma untreated or treated with 8 m urea were added to wells and incubated for 2 h at 37 °C. The binding studies were carried out by ELISA described as Materials and methods. The absorbance of collagen‐bound VNs was corrected using the reactivity of each VN to sheep anti‐VN IgGs. (B) The ratio of active VN in untreated plasma was obtained by dividing the collagen‐bound VNs in untreated plasma by the collagen‐bound VNs in urea‐treated plasma. Normal: blue bar; LC: orange bar. Data are presented as mean ± SD. *P < 0.05 compared to normal by t‐test. n = 10.