Figure 6.

Collagen binding of VNs in CCl₄‐treated plasma. (A) Collagen type I was immobilized on wells of a 96‐well plate. Control and CCl₄‐plasma untreated or treated with 8 m urea were added to wells and incubated for 2 h at 37 °C. The binding studies were performed by ELISA as described in Materials and methods. The absorbance of collagen‐bound VNs was corrected using the reactivity of each VN to sheep anti‐VN IgGs. (B) The ratio of collagen‐binding VNs in the untreated plasma was calculated from the binding activities before and after urea treatment of plasma. Control: green bar; CCl₄‐treated: yellow bar. Data are presented as mean ± SD. *P < 0.05 compared to control by Mann–Whitney U test. n = 4–8.