Table 1.
Lectin reactivity of VNs. The lectin reactivity of VNs illustrates the partial structures of glycans. The reactivity of each VN was measured with biotinyl lectins on the membrane and expressed as staining intensity. +, positive staining; ++, +++, positive staining with strong intensity; −, negative staining; NT, not tested
| Lectin | Human | Rat | Specificity | ||
|---|---|---|---|---|---|
| Normal | LC | Control | CCl4 | ||
| Con A | ++ | ++ | ++ | + | N‐linked biantennary or oligomannose type |
| RCA | ++ | ++ | +++ | + | Non‐reducing terminal Gal |
| DSA | + | ++ | + | ++ | Tri‐ or tetra‐antennary lactosamine type or (Galβ1‐4GlcNAc)n |
| LEL | − | − | + | ++ | Polylactosamine chain |
| AAL | + | ++ | + | + | Core or outer fucosyl residue |
| SNA | ++ | ++ | NT | NT | Sialylα2‐6 Gal |
| SNA* | − | − | NT | NT | |
| PVL | NT | NT | + | +++ | Tri‐ or tetra‐antennary sialyl α2‐3 or non‐reducing terminal GlcNAc |
| PVL* | NT | NT | − | − | |
*
Reactivity after desialylation.