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. 2019 Mar 26;13:281. doi: 10.3389/fnins.2019.00281

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Copyright © 2019 Wang, Lee, Kuei, Yao, Harrington, Bonaventure, Lovenberg and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double in situ hybridization of GPR139 (red) and DRD2 (white) mRNA in mouse mid brain. Yellow arrows indicate colocalization of GPR139 and DRD2 mRNA in the same cells, including periaqueductal gray (PAG, A), ventral tegmental area (VTA, C), and compact part of substantia nigra (SNC, E). Magnified images of PAG (B), VTA(D), and SNC (F) are shown with yellow arrows indicating example of colocalization signals. The nuclei of cells were stained with DAPI (blue). pc, posterior commissure; Aq, aqueduct; Dk, nucleus of Darkschewitsch; SNR, substantia nigra, reticular part.