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VPA promotes the maturation of neurons. Immunofluorescence staining of NeuN+ cells (red) in the SGZ of the DG; scale bar = 50 μm (Aa–d). Statistical analysis showed that there were no obvious differences between the VPA-treated mice and controls, P > 0.05 (B). Golgi–Cox-stained dendritic branches of neurons; scale bar = 25 μm (Ca,b). Analysis of spine density; the VPA-treated mice showed an obviously higher spine density than controls (D). ***P < 0.001.
