Fig. 1.

Single factor experiments. a Effect of solvent-to-flour ratio on protein extractability. b Effect of incubation time on protein extractability. c Effect of incubation temperature on protein extractability. d Effect of Hofmeister cationic salts on protein extractability. e Effect of Hofmeister cationic salts on protein extractability and protein precipitation. f Effect of Hofmeister anionic salts on protein extractability and protein precipitation. g Effect of NaCl concentration on protein extractability. h Effect of pH on protein extractability, i effect of dilution on protein precipitate yield upon extraction under RSM optimized conditions. Extraction conditions unless specified: Solvent-to-flour ratio 20:1, Incubation time 60 min, Ambient temperature, Salt concentration 0.25 M, Natural pH 5.5