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. 2019 Feb 20;41:357–369. doi: 10.1016/j.ebiom.2018.12.061

Fig. 1.

Fig. 1

Endogenous expression of TRIM25, pri-miR-3614, and miR-3614–3p in breast cancer (BC) tissues and BC-derived cell lines. (a) qRT-PCR was performed to examine TRIM25 mRNA expression in BC tissues versus non-tumor tissues. Data are shown as mean ± SEM (Student's t-test). (b) Representative IHC images of TRIM25 expression in non-tumor tissues and BC tissues. Magnification ×40. Scale bar, 50 μm. (c) TRIM25 mRNA and protein levels in BC cell lines (MCF-7, MDA-MB-231, HCC-1937 and MDA-MB-453) and normal human mammary gland cell line (HBL-100) were quantified by qRT-PCR and Western blot. (d, e) Pri-miR-3614 expression in BC tissues and cell lines. Data are shown as mean ± SEM (**P < 0.01, Student's t-test). (f, g) miR-3614-3p expression in BC tissues and cell lines. Data are presented as the mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, Student's t-test).