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. 2019 Feb 20;41:357–369. doi: 10.1016/j.ebiom.2018.12.061

Fig. 2.

Fig. 2

The expression of TRIM25, pri-miR-3614 and miR-3614-3p in response to estrogen (E2) and tamoxifen (TAM) treatment. (a–c) qRT–PCR analyses of TRIM25 mRNA, pri-miR-3614, and miR-3614-3p expression in MCF-7 and MDA-MB-231 cells treated with the indicated concentrations of E2 (0.1–100 nM) for 6 h. Data are shown as mean ± SEM (*P < 0.05, **P < 0.01, Student's t-test) (d) Western blot analyses of TRIM25 protein level in MCF-7 and MDA-MB-231 cells treated with E2 (0.1–100 nM). (e-g) qRT–PCR analyses of TRIM25 mRNA, pri-miR-3614 and miR-3614-3p levels in BC cells treated with E2 (100 nM) and the indicated concentrations of TAM (1–100 nM). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 (Student's t-test). (h) Western blot analyses of TRIM25 protein level in BC cells treated with E2 (100 nM) and TAM (1–100 nM) for 6 h.