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. 2019 Feb 20;41:357–369. doi: 10.1016/j.ebiom.2018.12.061

Fig. 6.

Fig. 6

MiR-3614-3p suppresses BC cell proliferation. (a, b) MTT cell proliferation assay and colony formation were performed at the indicated time points after transfection with pre-miR-3614, anti-miR-3614, or negative control. Data are presented as the mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, ANOVA analysis). (c) miR-3614-3p increases G1 phase arrest in BC cells. Flow cytometry analysis of BC cells transfected with pre-miR-3614, anti-miR-3614, or negative control treatment. Data are presented as the mean ± SEM. (*P < 0.05, **P < 0.01, ANOVA analysis). (d) The expression of CDK4, CyclinD1, and β-actin analyzed by Western blot after transfection with pre-miR-3614, anti-miR-3614, or negative control. (e) Xenograft studies show suppressed tumor growth when pre-miR-3614 is overexpressed in the transplanted cells. Gross morphology of tumors after 30 days of implantation with either LV-miR-3614 or LV-Ctrl cells (n = 3) (left). Tumor growth curves of the tumor volumes represent measurement taken every 3 d for 30 d (right). Data are shown as mean ± SEM (*P < 0.05, Student's t-test).