Fig. 5.

miR-873 suppressed the stemness through inactivating PI3K/Akt and ERK1/2 pathways in a PD-L1 dependent manner. (a, b) p-AKT and p-ERK levels were detected in MCF-7 and MDA-MB-231 cells with different treatment shown in labels. (c, d) The expression levels of p-AKT/p-ERK and stemness markers were examined in MCF-7 cells transfected with inhibitor-873 plus PI3K/AKT inhibitor (LY-294002) or ERK1/2 inhibitor (VX-11e), or plvx-PD-L1 plus LY-294002 or VX-11e, or not. (e, f) Spheroid formation or CD44+/CD24- sub-population was measured in MCF-7 cells depicted in (c). (***p < 0.001 vs Control group. ^###p < 0.001 vs plvx-PD-L1 or inhibitor-873). (Data were presented as the mean ± SD, n = 3).