Fig. 5.
Amplification of the butyryl-CoA:acetate CoA-transferase gene, the terminal enzyme of the central butyrate synthesis pathway of human gut microbiota, in fresh anaerobic conditions (ANO2), fresh aerobic conditions (O2), after one cycle of freezing and thawing in anaerobically processed specimens (FT1) or after heat-killing (HK) in PMA treated samples. Butyryl-coenzyme A(CoA) CoA transferase gene levels were measured relative to amplification in a 10-fold dilution series of neat faecal slurry (FS control). The dotted line represents limit of quantification of the assay. Box plots depict median and IQR and error bars depict minimum to maximum values of faecal slurry samples from 8 individual donors. Significant comparisons are indicated by stars. (*= p<0·05; **= p<0·01; ***= p<0·001; Wilcoxon matched-pairs signed rank test).