Fig. 4.

Levels of phosphorylated IκB-α, p38 and ERK with VWA domain treatment. U-937 macrophages were treated for 2, 4, 6, or 12 h with 5 μg/mL of His-MBP or His-MBP-VWA protein. The levels of phosphorylated IκB-α (A), p38 (B) and ERK (C) was then assessed by western blotting. The fold difference of each sample was compared against His-MBP treated macrophage at 1 h. Results were the means of 3 samples ± SEM. Significant fold differences from corresponding control values at the given timepoints are indicated by * (p < 0.05).