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. 2019 Feb 13;41:623–635. doi: 10.1016/j.ebiom.2019.02.020

Fig. 1.

Fig. 1

Consumption of diets differing in protein content resulted in substantial changes of ovarian primordial follicle activation. Ovaries from mice on diets with low protein (LP), normal protein (NP) or excess protein (HP) for 4 weeks, 12 weeks, 24 weeks and 48 weeks were analyzed for follicle counts at different developmental stages (n = 8–10 per group). (A) Representative ovarian histology of mice on diets for 4 weeks. #, primordial follicle. ##, primary follicle. Scale bars: 200 μm. Original magnification: ×100. Follicle counts at each developmental stage were determined at 4 weeks (B), 12 weeks (C), 24 weeks (D) and 48 weeks (E) on diets. (F) Ratio of primary to primordial follicles, based on primordial and primary follicle counts from B-E. Ovarian protein expressions of phosphorylated mTOR, S6, ERK1/2 and AMPK in mice on diets for 4 weeks (G) and 12 weeks (H) as detected by western blotting (n = 8 per group for quantification). Columns with different letter a,b,c denotes P < .05 by un-paired t-test. *P < .05, **P < .01, ***P < .001 by un-paired t-test.