Figure 1.
Whole body fluorescence imaging of AvBD7 in vivo and ex vivo post intraperitoneal injection in mice. Mice were injected with 100 μl of either H2O alone (negative control, left part of each image) or containing 100 μg of fluorescence-labeled AvBD7 or containing the same amount of free dye (according to absorbance measurement at 750 nm). Times for image acquisition are indicated on each image. (A) Mice imaged on left lateral view. (B) Pelleted peritoneal cells collected from control (left) or fluorescent AvBD7-injected mice. (C) Mice imaged on left lateral view, hiding the kidneys area. (D) Organs imaged ex vivo (K, kidneys; S, spleen; L, liver; MLN, mesenteric lymph node). (E) Quantification of AvBD7 fluorescence (black bars) by comparison to that of the free dye fluorescence (grey bars) in organs collected post i.p. injection in a sacrificed animal at three indicated times (in h). Values are given in million average radiant efficiency.