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. 2019 Feb 27;15:26–35. doi: 10.1016/j.omtn.2019.02.018

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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FPLC-Based Cellulose Chromatography of IVT mRNA

The total of 100 mg of a 2.2-kb-long U-containing IVT mRNA was purified by FPLC-based cellulose chromatography using chromatography buffer with 16% (v/v) ethanol, as described in the Materials and Methods. (A) The UV chromatogram of the FPLC purification run is shown. The collected fractions containing the eluted purified mRNA are marked as fractions 1–3, and the cellulose-bound RNA eluted with water is marked as fractions 4 and 5. (B) Analysis of the collected fractions by dot blot and electrophoresis on a 1.4% agarose gel.