Figure 6.

Translation of IVT mRNA Purified by Cellulose Chromatography

U- or m1Ψ-containing IVT mRNA encoding murine erythropoietin (mEPO) was purified either by two consecutive cycles using microcentrifuge spin columns filled with cellulose or by HPLC. (A) Unpurified, cellulose-, and HPLC-purified U- and m1Ψ-containing mEPO mRNAs (1.0 μg/dot) were analyzed in triplicates by dot blot using J2 dsRNA-specific mAb. (B) U- or m1Ψ-containing mEPO mRNAs that were unpurified and cellulose and HPLC purified were complexed with TransIT and intravenously injected into mice, and plasma mEPO levels were determined by ELISA at the indicated time. Data are represented as mean ± SD of the values obtained from 3 animals per group and are representative of three independent experiments.