Figure 5.

Alterations in Δψm of 8505C cells in vitro. (A) 8505C cells treated with 0, 2 or 4 µM SOV for 48 h prior to staining with JC-1. Δψm was detected by fluorescence microscopy. When Δψm was high, JC-1 accumulated in the mitochondrial matrix as a polymer (J-aggregates), producing red fluorescence; when Δψm was low, JC-1 accumulated in the mitochondrial matrix as JC-1 monomers (monomer), producing green fluorescence. (B) Representative histograms of 8505C cell labeling with JC-1 dye. Scale bar, 100 µm. ***P<0.001 vs. the control (0 µM SOV) group. SOV, sodium orthovanadate; Δψm, mitochondrial membrane potential.