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Analysis of MET + PTX-induced apoptosis by Annexin V/PI double-staining and Caspase-Glo 3/7 assays in prostate cancer cells. Cells were treated with MET (5 mM), PTX (10 nM for PC-3 cells, and 2 µM for 22RV1 and LNCaP cells) and MET + PTX for 24 h. (A) Flow cytometry was employed to analyze apoptotic cells. (B) Quantification of the percentage of apoptotic cells. (C) Caspase-3/7 activity was detected. *P<0.05, **P<0.01, ***P<0.001. MET, metformin; PI, propidium iodide; PTX, paclitaxel.
