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. 2019 Mar 6;17(5):4277–4284. doi: 10.3892/ol.2019.10119

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Western blot analysis of apoptosis-associated proteins in prostate cancer cells. Cells were treated with MET (5 mM), PTX (10 nM for PC-3 cells, and 2 µM for LNCaP cells) and MET + PTX for 24 h. (A) Western blot analysis was used to detect the expression of apoptosis-associated proteins. (B) Semi-quantification of western blotting. **P<0.01, ***P<0.001. Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; C3, caspase-3; C9, caspase-9; Clv, cleaved; Cyto-C, cytochrome c; MET, metformin; PARP, poly (ADP-ribose) polymerase; PTX, paclitaxel.