Fig. 6.

Schwann cell response to aLam. Number of Schwann cells (S-100 positive) and myelinated axons (toluidine blue) were similar between groups at both 2 and 10 wpi. G Ratio was similar between groups at 2 wpi but significantly reduced in the aLam group relative to PBS control at 10 wpi, indicative of thicker myelin sheath. A) Injured peroneal nerve showing Schwann cells (S-100, green; DAPI, blue) at and around the injury site; B) Bar graph showing similar S-100 fluorescence intensity (integrated density); C) Bar graphs showing similar S-100 positive cell counts between groups; D/E) Semi-thin cross-section of injured nerve treated with PBS (D) or aLam (E) stained with toluidine blue at 2 wpi; D) Bar graph showing similar counts of myelinated axons between groups at 2 and 10 wpi. G/H) Semi-thin cross-section of injured nerve treated with PBS (G) or aLam (H) stained with toluidine blue at 10 wpi; Scale bars in G/H represent 50 μm for D/E/G/H; I) Bar graph showing significantly reduced G Ratio of myelinated axons in the aLam group relative to PBS control at 10 wpi. Error bars represent SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)