Figure 2.

mRNA expression levels of CDCA4 in TNBC cells. (A) RT-qPCR. TNBC cells were grown and infected with a lentivirus carrying shCDCA4 or shCtrl and then subjected to RT-qPCR. CDCA4 mRNA expression in MDA-MB-468 cells was slightly decreased compared with that in the shCtrl group, whereas MDA-MB-231 cells exhibited a significant knockdown of CDCA4 expression (P<0.05). (B) Fluorescence microscopy. MDA-MB-231 cells infected with lentivirus-shCtrl and lentivirus-shCDCA4, were observed under a fluorescence and phase-contrast microscope. Fluorescence microscopy demonstrated that infection and green fluorescent protein expression rates were >80%. Magnification, ×100. The data were expressed as the means ± standard deviation. CDCA4, cell division cycle-associated protein 4; Ctrl, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; sh, short hairpin RNA; TNBC, triple negative breast cancer.